Acclimation and testing periods
Vendor Mice from the The Jackson Laboratory JAX® (Bar Harbor, ME) production facility were obtained and bled at TJL, and the frozen plasma were shipped and assayed at UWM Ages 9 wks of age Feed Most strains maintained on LabDiet 5K52 (6% fat) during the acclimation period Water Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA). Housing Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system. Bedding Steam autoclaved white pine shavings. Photoperiod 12 hours on (6 am to 6 pm), 12 hours off Relative humidity ~50% Temperature 24-25 °C Health status No pathogens detected in animal room. Mice appeared healthy during acclimation period. The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.