Acclimation & testing periods
Vendor Mice were obtained from The Jackson Laboratory (JAX®, Bar Harbor, ME) as part of a collaboration with the Mouse Phenome Project Ages 8-12 wks at the start of the experiment Feed 6% fat diet (Purina 5K52) Water Acidified water (pH 2.8-3.2) was made available ad libitum Housing Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system. Bedding Steam autoclaved white pine shavings Photoperiod 12 hr on 12 hrs off – 5:00 AM On – 5:00 PM Off Temperature 18 - 24°C Relative humidity ~50% Health status The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored. Regulatory compliance All housing and experimental procedures were conducted under the guidelines of the Canadian Council of Animal Care and were approved by the Biotechnology Research Institute (BRI) Animal Care Committee (Protocol number: 08-SEP-I-017) and the Animal Care Committee of McGill University (Protocol number: 5618).