Hematology automated analyzer: Sysmex Deutschland GmbH (XT-2000iV)
Mice (non-fasting) are anesthetized via isoflurane inhalation.
Blood is collected in the morning by retro-orbital puncture where 50 µL of whole blood is collected in an EDTA-coated 50 µL end-to-end capillary, diluted 1:5 with CellPack buffer, carefully mixed and placed on a rotary mixer for a minimun of 30 min.
Samples are kept at room temperature until analyzed.
Analysis is performed on the day of sample collection within 6h after sample collection. Samples with clots are not run.