This project is to investigate the relationships between genetic loci and different phenotypes influencing body weight. Two F2 populations were generated:
Cross 1 is an intercross of DBA/2 and NMRI8;
Cross 2 is an intercross of DBA/2 and DU6i. For both crosses
the F2 progeny were fed breeding diet (1) and phenotyped for body weight, muscle weight, fat weight and other related phenotypes (studied phenotypes vary in cross1 vs. cross2). For detailed protocols see Brockmann et al 2009.
(1) Breeding diet: containing 12.5 MJ/kg metabolic energy with an average content of 22.5% crude protein, 5.0% crude fat, 4.5% crude fiber, 6.5% crude ash, 13.5% water, 48.0% N-free extract, vitamins, trace elements, amino acids, and minerals (Altromin diet 1314).