Auwerx1 animal documentation

Diet effects on metabolic phenotypes in males of 55 BXD recombinant inbred strains of mice fed a high-fat diet   (2016)

Williams EG, Wu Y, Aebersold R, Auwerx J
With: Bou Sleiman M, Rapin A, Jha P, Dubuis S, Blattmann P, Argmann CA, Houten SM, Amariuta T, Wolski W, Zamboni N

Animal Documentation

Source of Mice: BXD mice strains were obtained from the University of Tennessee Health Science Center (Memphis, TN, USA)

Age (wks): 8-29 wks at testing

Breeding: Mice were bred for more than two generations at the École Polytechnique Fédérale de Lausanne (EPFL) animal facility before being incorporated into the study.

Test Facility: École Polytechnique Fédérale de Lausanne (EPFL) animal facility

Feed: Standard Rodent chow (CD) or High-fat diet (HFD) at 8 wks of age, ad libitum (see Procedure 1 for details of diets)

Water: Water ad libitum

Cage Type, Distributor, Material, Size: Isolator cages with individual air filtration

Husbandry: Cohorts are communally housed by strain and diet from birth until 23 weeks of age, and then individually housed until killed at 29 weeks of age

Temperature (°C): 22 +/- 2

Humidity (%): 50 +/- 10

Light / Dark Cycle: 12:12h

Care and Use Statement: All research was approved by the Swiss cantonal veterinary authorities of Vaud under licenses 2257 and 2257.1

Additional Notes: The study consisted of 80 cohorts of the BXD population from 41 strains, 41 on chow diet (CD) and 39 on high fat diet (HFD); with male mice from each strain separated into 2 groups of about 5 mice for each diet. Initially the study started with 201 CD and 185 HFD treated mice, of which 183 CD and 168 HFD mice survived before being killed, with all cohorts having 3 or more survivors except BXD56 which had 2 surviving individuals.